Pyronin Y
Pyronin Y (Ex-Max 544 nm/Em-Max 563 nm)
Pyronin Y is an intercalating cationic dye that has selectivity for RNA. It has an excitation peak at 548 nm and an emission peak at 566 nm. It is commonly used in fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 544 nm
- Emission maximum
- 563 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Molecular weight
- 302.8
Pyronin Y has an excitation maximum at 544 nm and an emission maximum at 563 nm, a Stokes shift of 19 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Pyronin Y?
- Pyronin Y is an intercalating cationic dye that has selectivity for RNA. It has an excitation peak at 548 nm and an emission peak at 566 nm. It is commonly used in fluorescence microscopy.
- What laser excites Pyronin Y?
- Pyronin Y is optimally excited by the 532 laser line. Its excitation maximum is 544 nm.
- What filter should I use to detect Pyronin Y?
- A 585/42 filter captures the peak of the Pyronin Y emission near 563 nm with minimal spillover.