Sytox Green
Sytox Green (Ex-Max 501 nm/Em-Max 521 nm)
Sytox Green from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 504 nm and an emission peak at 523 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 504 nm
- Emission maximum
- 523 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
Sytox Green has an excitation maximum at 504 nm and an emission maximum at 523 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Sytox Green?
- Sytox Green from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 504 nm and an emission peak at 523 nm.
- What laser excites Sytox Green?
- Sytox Green is optimally excited by the 488 laser line. Its excitation maximum is 504 nm.
- What filter should I use to detect Sytox Green?
- A 515/30 filter captures the peak of the Sytox Green emission near 523 nm with minimal spillover.