ATTO 495
ATTO 495 from ATTO-TEC is a novel fluorescent label derived from the well-known dye Acridine Orange. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye shows intense and long-lived phosphorescence in solid matrix or at low temperatures. ATTO 495 is moderately hydrophilic.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 527 nm
- Optimal laser line
- 488
- Relative brightness
- 2 of 5
- Extinction coefficient
- 80000
- Quantum yield
- .20 (PBS, pH 7.4)
- Fluorescence lifetime
- 1.0 ns
- Molecular weight
- 452
ATTO 495 has an excitation maximum at 495 nm and an emission maximum at 527 nm, a Stokes shift of 32 nm. Its extinction coefficient is 80000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is ATTO 495?
- ATTO 495 from ATTO-TEC is a novel fluorescent label derived from the well-known dye Acridine Orange. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye shows intense and long-lived phosphorescence in solid matrix or at low temperatures. ATTO 495 is moderately hydrophilic.
- What laser excites ATTO 495?
- ATTO 495 is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- How bright is ATTO 495?
- ATTO 495 has a relative brightness of 2 out of 5 (quantum yield .20).