PhenoVue ROS-490, Total Oxidative Stress Indicator
PhenoVue ROS-490, Total Oxidative Stress Indicator (Ex-Max 498 nm/Em-Max 517 nm)
PhenoVue™ ROS-490, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-490, Total Oxidative Stress Indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 498 nm and a maximum emission wavelength of 517 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 517 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
PhenoVue ROS-490, Total Oxidative Stress Indicator has an excitation maximum at 498 nm and an emission maximum at 517 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PhenoVue ROS-490, Total Oxidative Stress Indicator?
- PhenoVue™ ROS-490, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-490, Total Oxidative Stress Indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 498 nm and a maximum emission wavelength of 517 nm.
- What laser excites PhenoVue ROS-490, Total Oxidative Stress Indicator?
- PhenoVue ROS-490, Total Oxidative Stress Indicator is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- What filter should I use to detect PhenoVue ROS-490, Total Oxidative Stress Indicator?
- A 515/30 filter captures the peak of the PhenoVue ROS-490, Total Oxidative Stress Indicator emission near 517 nm with minimal spillover.