LysoBrite Green
LysoBrite Green (Ex-Max 497 nm/Em-Max 510 nm)
LysoBrite Green from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Green has an excitation peak at 497 nm and an emission peak at 510 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 510 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
LysoBrite Green has an excitation maximum at 497 nm and an emission maximum at 510 nm, a Stokes shift of 13 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is LysoBrite Green?
- LysoBrite Green from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Green has an excitation peak at 497 nm and an emission peak at 510 nm.
- What laser excites LysoBrite Green?
- LysoBrite Green is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect LysoBrite Green?
- A 515/30 filter captures the peak of the LysoBrite Green emission near 510 nm with minimal spillover.