Rhod-2 (Ca2+ bound)
Rhod-2 (Ca2+ bound) (Ex-Max 549 nm/Em-Max 574 nm)
Rhod-2, AM is a cell-permeant calcium indicator used in fluorescence microscopy. It is dim when unbound and increases with intensity upon binding calcium. It has an excitation peak at 549 nm and an emission peak at 574 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 549 nm
- Emission maximum
- 574 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Rhod-2 (Ca2+ bound) has an excitation maximum at 549 nm and an emission maximum at 574 nm, a Stokes shift of 25 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Rhod-2 (Ca2+ bound)?
- Rhod-2, AM is a cell-permeant calcium indicator used in fluorescence microscopy. It is dim when unbound and increases with intensity upon binding calcium. It has an excitation peak at 549 nm and an emission peak at 574 nm.
- What laser excites Rhod-2 (Ca2+ bound)?
- Rhod-2 (Ca2+ bound) is optimally excited by the 561 laser line. Its excitation maximum is 549 nm.
- What filter should I use to detect Rhod-2 (Ca2+ bound)?
- A 585/42 filter captures the peak of the Rhod-2 (Ca2+ bound) emission near 574 nm with minimal spillover.