BPE
BPE (Ex-Max 544 nm/Em-Max 563 nm)
B-PE, also known as B-Phycoerythrin, is a fluorescent protein produced by eukaryotic red algae like the genus Rhodella. B-PE consists of 3 subunits (alpha, beta and gamma). It has a primary excitation peak at 545 nm and a secondary peak at 563 nm. B-PE is closely related to R-PE which is the most common donor fluorophore in tandem dyes used in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 544 nm
- Emission maximum
- 563 nm
- Optimal laser line
- 488-561
- Optimal filter
- 585/42
- Relative brightness
- 5 of 5
- Extinction coefficient
- 2410000
- Quantum yield
- 0.98
- Molecular weight
- 240000
BPE has an excitation maximum at 544 nm and an emission maximum at 563 nm, a Stokes shift of 19 nm. Its extinction coefficient is 2410000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is BPE?
- B-PE, also known as B-Phycoerythrin, is a fluorescent protein produced by eukaryotic red algae like the genus Rhodella. B-PE consists of 3 subunits (alpha, beta and gamma). It has a primary excitation peak at 545 nm and a secondary peak at 563 nm. B-PE is closely related to R-PE which is the most common donor fluorophore in tandem dyes used in flow cytometry.
- What laser excites BPE?
- BPE is optimally excited by the 488-561 laser line. Its excitation maximum is 544 nm.
- What filter should I use to detect BPE?
- A 585/42 filter captures the peak of the BPE emission near 563 nm with minimal spillover.
- How bright is BPE?
- BPE has a relative brightness of 5 out of 5 (quantum yield 0.98).