CF532
CF®532 (Ex-Max 527 nm/Em-Max 558 nm)
CF®532 from Biotium is a fluorophore with an excitation peak at 527 nm and an emission peak at 558 nm. It is spectrally similar to Alexa Fluor™ 532. Due to its unique spectral characteristics, it may be useful in spectral flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 527 nm
- Emission maximum
- 558 nm
- Optimal laser line
- 532
- Optimal filter
- 530/43
- Relative brightness
- 1 of 5
- Extinction coefficient
- 96000
- Molecular weight
- 685
- Commercial conjugates
- 7 from 1 supplier
CF532 has an excitation maximum at 527 nm and an emission maximum at 558 nm, a Stokes shift of 31 nm. Its extinction coefficient is 96000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CF532?
- CF®532 from Biotium is a fluorophore with an excitation peak at 527 nm and an emission peak at 558 nm. It is spectrally similar to Alexa Fluor™ 532. Due to its unique spectral characteristics, it may be useful in spectral flow cytometry.
- What laser excites CF532?
- CF532 is optimally excited by the 532 laser line. Its excitation maximum is 527 nm.
- What filter should I use to detect CF532?
- A 530/43 filter captures the peak of the CF532 emission near 558 nm with minimal spillover.
- How bright is CF532?
- CF532 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF532?
- FluoroFinder tracks 7 commercially available CF532 conjugates, searchable by target, clone, host and application.