VivaFix 547/573
VivaFix 547/573 (Ex-Max 547 nm/Em-Max 573 nm)
VivaFix 547/573 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 547 nm and an emission peak at 573 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 547 nm
- Emission maximum
- 573 nm
- Optimal laser line
- 532
- Optimal filter
- 585/40
VivaFix 547/573 has an excitation maximum at 547 nm and an emission maximum at 573 nm, a Stokes shift of 26 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is VivaFix 547/573?
- VivaFix 547/573 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 547 nm and an emission peak at 573 nm.
- What laser excites VivaFix 547/573?
- VivaFix 547/573 is optimally excited by the 532 laser line. Its excitation maximum is 547 nm.
- What filter should I use to detect VivaFix 547/573?
- A 585/40 filter captures the peak of the VivaFix 547/573 emission near 573 nm with minimal spillover.